A simple, fast and precise reverse phase high performance liquid chromatographic method has been developed for the estimation of spironolactone in oral suspension. The chromatographic separation was achieved on zorbax RX-C18 (150 x 4.6) mm; 5 µm column with an isocratic mixture of methanol: water (60:40). The mobile phase at a flow rate of 1 ml/min, injection volume 20 μl and wavelength of detection was kept at 240 nm with column temperature at 25°C. The retention times for spironolactone, methyl paraben and propyl paraben were 6.61±0.04 min, 2.25±0.04 min and 4.67±0.04 min respectively. Here methyl paraben and propyl paraben is a major excipient of oral solution. The linearity was obtained in the range of 250-600 μg/ml, 50-120 μg/ml and 5-12 μg/ml for spironolactone, methyl paraben and propyl paraben respectively with correlation coefficient 0.998, 0.999 and 0.999 for spironolactone, methyl paraben and propyl paraben respectively. The proposed methods were validated as per ICH guidelines and successfully applied for the determination of investigated drugs in oral suspension.
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